Tear Film and Dry Eye

Our interests and expertise:

  • Development of in vitro models
  • Dry eye disease
  • Live imaging of cell morphology
  • Ocular response to contact lens wear
  • Tear film characterization (physical and chemical)
  • Tissue assessment, including the meibomian glands and lid wiper

Clinical capabilities:

  • Assessment of lid wiper epitheliopathy
  • Biomicroscopic grading with photo and video documentation
  • Confocal microscopy (in vivo)
  • Development of subjective tools and rating scales
  • Interferometry
  • Measurement of lipid layer appearance and thickness
  • Meibography
  • Tear film break-up time, drainage kinetics, meniscus height, volumetric analysis
  • Use of diagnostic stains

Laboratory capabilities

  • Analysis of inflammatory markers
  • Cell culture
  • Cell identification, quantification and viability
  • Characterization of tear film neutrophil phenotypes
  • Confocal microscopy
  • Cytology and immunohistochemistry
  • High-sensitivity radioactive biochemical assays
  • Meibum analysis
  • Osmolality
  • Quantification and characterization of proteins and lipids
  • Tear ferning
  • Tear film stability