Muntz,A., van Doorn,K., Subbaraman,L. N., Jones,L. W. Impression cytology of the lid wiper area. Journal of Visualized Experiments 2016;2016,114:. [ Show Abstract ]
Few reports on the cellular anatomy of the lid wiper (LW) area of the inner eyelid exist and only one report makes use of cytological methods. The optimization of a method of collecting, staining and imaging cells from the LW region using impression cytology (IC) is described in this study. Cells are collected from the inner surface of the upper eyelid of human subjects using hydrophilic polytetrafluoroethylene (PTFE) membranes, and stained with cytological dyes to reveal the presence of goblet cells, mucins, cell nuclei and various degrees of pre- and parakeratinization. Immunocytochemical dyes show cell esterase activity and compromised cell membranes by the use of a confocal scanning laser microscope. Up to 100 microscopic digital images are captured for each sample and stitched into a high-resolution, large scale image of the entire IC span. We demonstrate a higher sensitivity of IC than reported before, appropriate for identifying cellular morphologies and metabolic activity in the LW area. To our knowledge, this is the first time this selection of fluorescent dyes was used to image LW IC membranes. This protocol will be effective in future studies to reveal undocumented details of the LW area, such as assessing cellular particularities of contact lens wearers or patients with dry eye or lid wiper epitheliopathy. Â© 2016 Journal of Visualized Experiments.
Muntz,A., Subbaraman,L. N., Sorbara,L., Jones,L. Tear exchange and contact lenses: A review. Journal of Optometry 2015;8,1:2-11. [ Show Abstract ]
Tear exchange beneath a contact lens facilitates ongoing fluid replenishment between the ocular surface and the lens. This exchange is considerably lower during the wear of soft lenses compared with rigid lenses. As a result, the accumulation of tear film debris and metabolic by-products between the cornea and a soft contact lens increases, potentially leading to complications. Lens design innovations have been proposed, but no substantial improvement in soft lens tear exchange has been reported. Researchers have determined post-lens tear exchange using several methods, notably fluorophotometry. However, due to technological limitations, little remains known about tear hydrodynamics around the lens and, to-date, true tear exchange with contact lenses has not been shown. Further knowledge regarding tear exchange could be vital in aiding better contact lens design, with the prospect of alleviating certain adverse ocular responses. This article reviews the literature to-date on the significance, implications and measurement of tear exchange with contact lenses.
Liu,S., Chang,C. N., Verma,M. S., Hileeto,D., Muntz,A., Stahl,U., Woods,J., Jones,L. W., Gu,F. X. Phenylboronic acid modified mucoadhesive nanoparticle drug carriers facilitate weekly treatment of experimentallyinduced dry eye syndrome. Nano Research 2015;8,2:621-635.
Muntz A, Subbaraman L, Jones L. Is there an association between lid wiper epitheliopathy, lens type and contact lens discomfort?. Optom Vis Sci 2016;93: E-abstract 160047.
Muntz A, vanDoorn K, Subbaraman L, Jones L. Impression cytology of the lid wiper area. Invest Ophthalmol Vis Sci 2015;56: E-abstract 4432. [ PDF ]
Liu S, Chang C, Verma M, Hileeto D, Muntz A, Stahl U, Woods J, Jones L, Gu F. Phenylboronic acid modified mucoadhesive nanoparticles facilitate weekly treatment of dry eye syndrome. Invest Ophthalmol Vis Sci 2014;55: E-abstract 2160.
Muntz A, Lorentz H, Walther H, Heynen M, Joyce E, Sickenberger W, Jones L. Utility of a pulsating contact lens case to aid cholesterol removal from contact lens materials soaked in a no-rub MPS regimen. Contact Lens & Anterior Eye 2011;34,S1:S9.